Composite

Part:BBa_K4257022:Design

Designed by: Ye Gu   Group: iGEM22_CPU_Nanjing   (2022-09-26)


RPD+PPK-M


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequential configuration of RPD and PPK-M in expression vector should be considered. An independent RBS site was placed in front of PPK-M to enhance its expression efficiency.

Source

This composite Part was commercially synthesized base on the sequences of Parts RPD, RBS (Ribosome Binding Sites), and PPK-M.

References

Hirota, R., Yamane, S.-t., Fujibuchi, T., Motomura, K., Ishida, T., Ikeda, T. and Kuroda, A. (2012) Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506. Journal of Bioscience Bioengineering 113(4), 445-450.

Rudat, A.K., Pokhrel, A., Green, T.J. and Gray, M. (2018) Mutations in Escherichia coli polyphosphate kinase that lead to dramatically increased in vivo polyphosphate levels. Journal of Bacteriology 200(6), e00697-00617.