Part:BBa_K4256666:Experience
The 2*2 Bistable-switch (Bluet BS)
To verify the feasibility of the switch, The UCAS-China iGEM team 2022 designed two plasmids (pET-28a) containing our switch sequences. With four different fluorescent proteins added as reporters, we can quantify the feasibility of our design. In the absence of blue light, YFP (yellow) and EYFP (cyan) proteins will be produced at 37°C and 25°C respectively. In contrast upon blue light excitation, the reporter located at the other side of the switch will separately fabricate mCherry (red) and JGFP (green) proteins at 37°C and 25°C. We managed to transform our plasmids into the competent cell (DH5) and successfully observed the expression of the report proteins through the fluorescence microscope (Results are as followed).
Figure 1a. the result of the bistable-switch.
Due to the coronavirus pandemic and inadequate staffing, we only validate the practicability of each pathway in our switch. The changes between different statuses of our switch have not been completely texted. Within our design, the switch should be changed along with the existence of the blue light. ===Applications of BBa_K4256666=== ===User Reviews===