![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
BluetL-BS
Part:BBa_K4256666:Design
Designed by: KaMing Li Group: iGEM22_UCAS-China (2022-10-09)
BluetL (Blue light & Low Temperature) Bistable-Switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1620
Illegal NheI site found at 1733
Illegal NheI site found at 1756 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2418
Illegal BamHI site found at 111 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To verify the feasibility of the switch, The UCAS-China iGEM team 2022 designed two plasmids (pET-28a) containing our switch sequences. With four different fluorescent proteins added as reporters, we can quantify the feasibility of our design. In the absence of blue light, YFP (yellow) and EYFP (cyan) proteins will be produced at 37°C and 25°C respectively. In contrast upon blue light excitation, the reporter located at the other side of the switch will separately fabricate mCherry (red) and JGFP (green) proteins at 37°C and 25°C.
Source
BBa_K3247005 BBa_K2469004