Composite

Part:BBa_K4256166

Designed by: Jingke Wang   Group: iGEM22_UCAS-China   (2022-09-29)


ccdB toxin expression system

BBa_K4256166 provides a stable native expression of the toxin protein ccdB for the suicide switch.The ccdB sequence in BBa_K4256166, located downstream of the T7 promoter, allows for the sustained and stable expression of the toxin protein. ccdB toxin prevents the DNA gyrase from functioning, which leads to double-strand breaks in DNA, but its toxic effect can be inhibited by the antitoxin protein ccdA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 158
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 119
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 158
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 158
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None