Composite

Part:BBa_K4256155

Designed by: Jingke Wang   Group: iGEM22_UCAS-China   (2022-09-29)


Oxygen-sensitive ccdA antitoxin expression system

BBa_K4256155 is an expression system of the antitoxin protein ccdA (BBa_K4256111) under the control of the oxygen-controlled promoter HIP-1 (BBa_K4256100). Under anaerobic conditions，ccdA is normally expressed and inhibits the killing effect of ccdB; under normoxic conditions ccdA expression is repressed.Under hypoxic conditions, the stable presence of the [4Fe-4S] cluster in the FNR protein drives the FNR protein to form a homodimer, and this dimer can induce the initiation of downstream ccdA expression; under normoxic conditions, the [4Fe-4S] cluster is disassembled, and the FNR dimer is depolymerized, so the initiation of ccdA transcription is inhibited.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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