Coding

Part:BBa_K4256007

Designed by: Yuqi Liu   Group: iGEM22_UCAS-China   (2022-09-29)


ech (enoyl-CoA hydratase/aldolase) from Streptomyces sp. V-1

This is the coding sequence of ech, encoding enoyl-CoA hydratase/aldolase, from Streptomyces sp. V-1. This enzyme converts feruloyl-CoA to 4-hydroxy-3-methoxyphenyl-β-hydroxypropionyl-CoA, and converts 4-hydroxy-3-methoxyphenyl-β-hydroxypropionyl-CoA to vanillin. Accession: KC847406. Reference: Yang,W., Tang,H., Ni,J., Wu,Q., Hua,D., Tao,F. and Xu,P. Characterization of two streptomyces enzymes that convert ferulic Acid to vanillin. PLoS ONE 8 (6), E67339 (2013).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 544
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None