Coding

Part:BBa_K4256005

Designed by: Yue Lu   Group: iGEM22_UCAS-China   (2022-09-27)


comt (caffeate O-methyltransferase) from A. thaliana (optimized)

The original sequence is from Arabidopsis thaliana (thale cress), encoding O-methyltransferase 1. Caffeate O-methyltransferase can convert caffeic acid to ferulic acid. To synthesize this enzyme in E. coli, the sequence was codon-optimized by GenSmart. Reference: Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, etc. Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. Nature. 2000 Dec 14;408(6814):823-6.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
    Illegal BamHI site found at 498
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None