Regulatory
PR

Part:BBa_K4252025:Design

Designed by: Jiajie Zhang   Group: iGEM22_HNU_China   (2022-10-11)


R promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter is regulated by cI857 and inhibited when cI857 forms dimer.

Source

gtgcgtgttgactattttacctctggcggtgataatggttgcatgtactaaggaggttg

References

Wang X, Han JN, Zhang X, Ma YY, Lin Y, Wang H, Li DJ, Zheng TR, Wu FQ, Ye JW, Chen GQ. Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli. Nat Commun. 2021 Mar 3;12(1):1411. doi: 10.1038/s41467-021-21654-x. PMID: 33658500; PMCID: PMC7930084.