RNA

Part:BBa_K4245004:Design

Designed by: Logan Bachman, Manasvi Gupta, Natalie Tan, Vandana Vijay Kumar, Christina Cho, Nick Lockhart, Hari Mudigonda, Janet Standeven   Group: iGEM22_Lambert_GA   (2022-08-17)


Upstream tRNA Scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The scaffold sequence was taken from the basic part BBa_K734002 (Spinach Aptamer with Stabilizing tRNA Scaffold).

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339