Composite

Part:BBa_K4245002:Design

Designed by: Manasvi Gupta, Natalie Tan, Vandana Vijay Kumar, Christina Cho, Nick Lockhart, Hari Mudigonda, Janet Standeven   Group: iGEM22_Lambert_GA   (2022-08-17)


iSpinach aptamer with LacI repression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 375
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part works identically to BBa_K4245001, a composite part producing Spinach, and BBa_K4245003, a composite part producing iSpinach-D5-G30-A32; the only difference is the aptamer produced. This disparity allows accurate comparison of the basic aptamer parts.

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083

Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339