Part:BBa_K4244008
Dismed2EnvZ chimeric sensor of two component system
Dismed2EnvZ is a chimeric sensor part of a two-component system (TCS). In specific, the TCS consists of a chimeric sensor formed by Dismed2 that is the extracellular domain of RetS, which is a sensor protein from Pseudomonas aeruginosa known to sense mucin (1). The transmitter domain of the chimeric sensor is composed of HisKA and HATPase domains EnvZ. EnvZ is part of the popular E. coli-derived two-component system EnvZ-OmpR (2,3). The EnvZ-OmpR TCS functions in such a way that EnvZ, when activated, phosphorylates the transcription factor OmpR, which subsequently activates the OmpC promoter. In our design OmpC regulates the transcription of the essential gene gmk, making our organism dependent on mucin.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 271
Illegal PstI site found at 804 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 271
Illegal PstI site found at 804 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 271
Illegal PstI site found at 804 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 271
Illegal PstI site found at 804
Illegal NgoMIV site found at 69
Illegal NgoMIV site found at 88
Illegal NgoMIV site found at 535
Illegal NgoMIV site found at 1002
Illegal NgoMIV site found at 1042 - 1000COMPATIBLE WITH RFC[1000]
references
1. Wang BX, Cady KC, Oyarce GC, Ribbeck K, Laub MT. Two-Component Signaling Systems Regulate Diverse Virulence-Associated Traits in Pseudomonas aeruginosa. Appl Environ Microbiol. 2021 May 11;87(11).
2. Ganesh I, Vidhya S, Eom GT, Hong SH. Construction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System. J Microbiol Biotechnol. 2017 Jun 28;27(6):1106–11.
3. Kenney LJ, Anand GS. EnvZ/OmpR Two-Component Signaling: An Archetype System That Can Function Noncanonically. EcoSal Plus. 2020 Mar 20;9(1).
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