Part:BBa_K4244006

OmpC

OmpC is a porin gene, whose promoter is controlled by the transcription factor OmpR, part of the EnvZ/OmpR two component system. The EnvZ/OmpR two-component system in normal conditions controls the expression of the ompC and ompF porin genes, controlling iron homeostasis (1). This part is native to </i>Escherichia coli</i>, which means that it has directly been amplified from the organism that we are working on: </i>E. coli</i> Nissle 1917. Since this part was originally upstream the OmpC gene, it also contains its native rbs. E. coli OmpC without rbs is under the iGEM registry code BBa_K769001.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Gerken H, Vuong P, Soparkar K, Misra R. Roles of the EnvZ/OmpR Two-Component System and Porins in Iron Acquisition in Escherichia coli. mBio. 2020;11(3):e01192-20. Published 2020 Jun 23. doi:10.1128/mBio.01192-20