Reporter

Part:BBa_K4242019:Design

Designed by: zhelin Jiang   Group: iGEM22_CUG-China   (2022-09-29)


RBS+FleQ+TT+Pci+Pbad+RBS+gfp+TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2837
    Illegal XhoI site found at 886
    Illegal XhoI site found at 1339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 279
    Illegal NgoMIV site found at 1287
    Illegal NgoMIV site found at 1299
    Illegal NgoMIV site found at 1375
    Illegal NgoMIV site found at 1460
    Illegal AgeI site found at 688
    Illegal AgeI site found at 2672
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3572
    Illegal SapI site found at 2654
    Illegal SapI.rc site found at 301
    Illegal SapI.rc site found at 1234
    Illegal SapI.rc site found at 1243


Design Notes

This is a composite part that use GFP to detect the function of FleQ in the plasmid.


Source

Use RBS to produce FleQ protein and use the GFP protein under the control of Pci and Pbad in the plasmid to reflect FleQ protein working.


References