Reporter
Part:BBa_K4242019:Design
Designed by: zhelin Jiang Group: iGEM22_CUG-China (2022-09-29)
RBS+FleQ+TT+Pci+Pbad+RBS+gfp+TT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2837
Illegal XhoI site found at 886
Illegal XhoI site found at 1339 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 279
Illegal NgoMIV site found at 1287
Illegal NgoMIV site found at 1299
Illegal NgoMIV site found at 1375
Illegal NgoMIV site found at 1460
Illegal AgeI site found at 688
Illegal AgeI site found at 2672 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3572
Illegal SapI site found at 2654
Illegal SapI.rc site found at 301
Illegal SapI.rc site found at 1234
Illegal SapI.rc site found at 1243
Design Notes
This is a composite part that use GFP to detect the function of FleQ in the plasmid.
Source
Use RBS to produce FleQ protein and use the GFP protein under the control of Pci and Pbad in the plasmid to reflect FleQ protein working.