Reporter
Part:BBa_K4242015:Design
Designed by: zhelin Jiang Group: iGEM22_CUG-China (2022-09-29)
Pbad+RBS+fleN+RBS+fleQ+TT+Pci+Ppel+RBS+gfp+TT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal XhoI site found at 2972
Illegal XhoI site found at 3425 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2000
Illegal NgoMIV site found at 2365
Illegal NgoMIV site found at 3373
Illegal NgoMIV site found at 3385
Illegal NgoMIV site found at 3461
Illegal NgoMIV site found at 3546
Illegal AgeI site found at 979
Illegal AgeI site found at 2774 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4596
Illegal SapI site found at 961
Illegal SapI.rc site found at 2387
Illegal SapI.rc site found at 3320
Illegal SapI.rc site found at 3329
Design Notes
This is a composite part that uses GFP to detect the function of FleN and FleQ in the plasmid.
Source
Use RBS to produce FleN protein and FleQ protein under the promoter Pbad and use the GFP protein under the control of Pci and Ppel in the plasmid to reflect FleN and FleQ cooperation.