Generator

Part:BBa_K4242010

Designed by: zongheng Wei   Group: iGEM22_CUG-China   (2022-09-29)


Pci+RBS+fleQ+TT

Uses promoter Pci(BBa_R0051), RBS(BBa_J34801), protein FleQ coding region(BBa_K4242000) and TT(BBa_B0015). This part is a device to construct the expression of protein FleQ that control by promoter Pci. FleQ is a transcription factor combined with c-di-GMP that can regulate genes about the expression of flagella biosynthesis and exopolysaccharide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 943
    Illegal XhoI site found at 1396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 336
    Illegal NgoMIV site found at 1344
    Illegal NgoMIV site found at 1356
    Illegal NgoMIV site found at 1432
    Illegal NgoMIV site found at 1517
    Illegal AgeI site found at 745
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 358
    Illegal SapI.rc site found at 1291
    Illegal SapI.rc site found at 1300


[edit]
Categories
Parameters
None