Coding

Part:BBa_K4242003:Design

Designed by: zhelin Jiang   Group: iGEM22_CUG-China   (2022-09-29)


FleN gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design this part as a protein coding region.


Source

Pseudomonas aeruginosa, the sequence is found through Genbank https://www.ncbi.nlm.nih.gov/gene/881866

References

[1]Baraquet C, Harwood CS. Cyclic diguanosine monophosphate represses bacterial flagella synthesis by interacting with the Walker A motif of the enhancer-binding protein FleQ. Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18478-83.

[2]Baraquet C, Murakami K, Parsek MR, Harwood CS. The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP. Nucleic Acids Res. 2012 Aug;40(15):7207-18.