Coding

Part:BBa_K4241797

Designed by: Vicera Christian Nelson Tejedor   Group: iGEM22_HKU_HongKong   (2022-10-11)


Ulp1_Active Domain (Microalgae)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Overview

This is the active domain of Ulp1 used in SUMO-cleavage. Codon optmized for microalgal expression systems.

Usage and Biology

This part is the active domain of Ulp1 used for cleavage of SUMO-tags.
This is a coding sequence only to be used after a promoter and translation initiation region. This is also codon optimized for Chlorella vulgaris and other microalgal expression systems.




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Categories
Parameters
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