Terminator

Part:BBa_K4241794

Designed by: Vicera Christian Nelson Tejedor   Group: iGEM22_HKU_HongKong   (2022-10-10)


Terminator.png

TRAmy3D

  • Rice Amylase 3D terminator sequence. To be used together with CvNDI1 or CvNDI2

Overview

Rice Amylase 3D terminator sequence. To be used together with CvNDI1 and CvNDI. This part is derived from the following paper: https://www.nature.com/articles/s41598-020-69620-9

Usage and Biology

This terminator is to be used in tandem with CvNDI1 and CvNDI2 inducible promoters. This system is a strong promoter expression system induced via nitrogen starvation.

References

Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9

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