Regulatory

Part:BBa_K4241791:Design

Designed by: Vicera Christian Nelson Tejedor   Group: iGEM22_HKU_HongKong   (2022-10-10)


Nitrogen Starvation Inducible promoter 2 (Microalgae)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 87
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 238
    Illegal XhoI site found at 663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 630
    Illegal PstI site found at 679
    Illegal PstI site found at 749
    Illegal NgoMIV site found at 902
    Illegal AgeI site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Inducible promoter activated via nitrogen starvation


Source

Chlorella vulgaris genomic

References