Coding

Part:BBa_K4234000:Design

Designed by: Robert Kaptur   Group: iGEM22_Duke   (2022-10-11)


IDH1 R132H mutation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 253
    Illegal BglII site found at 11
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 253
    Illegal NgoMIV site found at 1105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 970
    Illegal BsaI.rc site found at 1093
    Illegal SapI.rc site found at 777


Design Notes

The part requires a promoter, terminator and Kozak sequence to be expressed in eukaryotic systems.


Source

This sequence comes from Homo sapiens

References