Plasmid
CueO-PET28

Part:BBa_K4232008:Design

Designed by: Songyuan Kong   Group: iGEM22_BIT-China   (2022-10-03)


A plasmid used to catalyze oxygen and reducing substances


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1751
    Illegal XbaI site found at 1894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1751
    Illegal NheI site found at 1790
    Illegal NotI site found at 1724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1751
    Illegal BglII site found at 1960
    Illegal BamHI site found at 1757
    Illegal XhoI site found at 1717
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1751
    Illegal XbaI site found at 1894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1751
    Illegal XbaI site found at 1894
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 1992
    Illegal NgoMIV site found at 3580
    Illegal NgoMIV site found at 3740
    Illegal NgoMIV site found at 6783
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4657


Design Notes

NONE


Source

Laccase comes from E. coli and pet28a is obtained by procurement

References