Regulatory
nirb

Part:BBa_K4232002:Design

Designed by: Songyuan Kong   Group: iGEM22_BIT-China   (2022-09-22)


A oxygen-sensitive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 409
    Illegal BglII site found at 1278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1849
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2396
    Illegal SapI.rc site found at 68
    Illegal SapI.rc site found at 2351


Design Notes

Use under conditions of no nitrate interference


Source

nirb sequence found through Genbak:https://www.ncbi.nlm.nih.gov/gene/947868

References