Composite

Part:BBa_K4229078:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-10-09)


SPD5 with SnoopTag and SpyTag regulated by lamdapL promotor and LacI promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 443
    Illegal EcoRI site found at 2862
    Illegal EcoRI site found at 4036
    Illegal SpeI site found at 1840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 443
    Illegal EcoRI site found at 2862
    Illegal EcoRI site found at 4036
    Illegal SpeI site found at 1840
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 443
    Illegal EcoRI site found at 2862
    Illegal EcoRI site found at 4036
    Illegal BglII site found at 452
    Illegal BglII site found at 2763
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 443
    Illegal EcoRI site found at 2862
    Illegal EcoRI site found at 4036
    Illegal SpeI site found at 1840
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 443
    Illegal EcoRI site found at 2862
    Illegal EcoRI site found at 4036
    Illegal SpeI site found at 1840
    Illegal NgoMIV site found at 335
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

...


Source

Is an improved version of BBa_K3009033, and was cloned out of it.

=References

1] S. Alberti, “Phase separation in biology,” 2017, doi: 10.1111/pbi.1280.

[2] M. C. Huber et al., “Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments,” Nat Mater, vol. 14, no. 1, pp. 125–132, Jan. 2015, doi: 10.1038/nmat4118.