Coding

Part:BBa_K4229074:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-10-04)


BMC-T1_Y96TAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 706
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 36
    Illegal AgeI site found at 510
    Illegal AgeI site found at 618
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The plasmid containing this protein was kindly sent to us by the Kerfeld group.

References

H. Chen, J. Wilson, S. Ottinger, Q. Gan, and C. Fan, “Introducing noncanonical amino acids for studying and engineering bacterial microcompartments,” Curr. Opin. Microbiol., vol. 61, pp. 67–72, 2021, DOI: 10.1016/j.mib.2021.03.004.

Q. Gan, B. P. Lehman, T. A. Bobik, and C. Fan, “Expanding the genetic code of Salmonella with non-canonical amino acids,” Sci. Rep., vol. 6, no. November, pp. 1–7, 2016, DOI: 10.1038/srep39920.