Composite

Part:BBa_K4229055:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)


otsB under the regulation of T7 promotor and Lac operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 529
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

7 promotor and LacI operator are the natural regulators of the MCS of pET-Duet-1. otsB was cloned out the genomic DNA of e.Coli. And was cloned inside of pET-Duet-1, which was given to us by the CIBBS Toolbox from the Uni Freiburg.

References

He Li et al.: Enhanced production of trehalose in Escherichia coli by homolougous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarty, JBB, Vol. 113 No. 2, 224-232, 2012