![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4229049:Design
Designed by: Nikita Edel Group: iGEM22_Freiburg (2022-09-29)
"Full wiffelball" with tags, under regulation of lambdapLhybrid promotor and LacI promotor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 444
Illegal PstI site found at 1711 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 444
Illegal PstI site found at 1711
Illegal NotI site found at 1515
Illegal NotI site found at 2892 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 444
Illegal BglII site found at 453 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 444
Illegal PstI site found at 1711 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 444
Illegal PstI site found at 1711
Illegal NgoMIV site found at 335
Illegal NgoMIV site found at 842
Illegal NgoMIV site found at 2512
Illegal NgoMIV site found at 2868
Illegal AgeI site found at 1319
Illegal AgeI site found at 1427
Illegal AgeI site found at 2014 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
none
Source
The plasmid containing this protein was kindly sent to us by the Kerfeld group.
References
H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.
H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.