Composite

Part:BBa_K4229045:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)


H,T1 with Snoop and SpyTag,T2,T3 proteins assemble the "full wiffelball"


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1253
    Illegal NotI site found at 1057
    Illegal NotI site found at 2434
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1253
    Illegal NgoMIV site found at 384
    Illegal NgoMIV site found at 2054
    Illegal NgoMIV site found at 2410
    Illegal AgeI site found at 861
    Illegal AgeI site found at 969
    Illegal AgeI site found at 1556
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Was kindly send to us by the Kerfeld group.

References

[1] C. A. Kerfeld, C. Aussignargues, J. Zarzycki, F. Cai, and M. Sutter, “Bacterial microcompartments,” Nat. Rev. Microbiol., vol. 16, no. 5, pp. 277–290, 2018, doi: 10.1038/nrmicro.2018.10.

[2] H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.