Coding

Part:BBa_K4229030:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)


T1-Protein from the synthetic wiffleball (dreived from HO-Shell)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 517
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 36
    Illegal AgeI site found at 321
    Illegal AgeI site found at 429
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The plasmid containing this protein were kindly send to us by the Kerfeld group.

References

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, doi: 10.1186/s12915-019-0691-z.