![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4229016:Design
Designed by: Nikita Edel Group: iGEM22_Freiburg (2022-09-28)
RBS-snoopCatcherTnaA-RBS-FRE
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 990
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 990
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 990
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 990
Illegal AgeI site found at 155
Illegal AgeI site found at 241
Illegal AgeI site found at 1613 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2150
Illegal SapI.rc site found at 2270
Design Notes
none
Source
e.Coli (MG1655 strain). This biobrick itself was synthetised. The use was taken out of a paper.
References
Yin H. et.al., "Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli", ACS OMEGA, vol.6, 20569−20576, 2021, https://doi.org/10.1021/acsomega.1c02679.