Coding

Part:BBa_K4229015:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)


spyCatcherXiaI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 879
    Illegal AgeI site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Was synthesized as a fusion protein. XiaI is a gene out of Streptomyces sp. But the fusion with the spyCatcher (BBa_K4229010) was designed by us.

References

Yin H. et.al., "Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli", ACS OMEGA, vol.6, 20569−20576, 2021, https://doi.org/10.1021/acsomega.1c02679.

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, doi: 10.1186/s12915-019-0691-z.