Part:BBa_K4228001

promoter

Promoter is one of the many key factors for the expression efficiency, and the expression level of proteins could be regulated by controlling transcript production at the promoter level. Generally, promoters used for the expression of recombinant proteins in B. subtilis mainly include inducible promoters, stress-specific promoters, auto-inducible (self-inducible) promoters, and constitutive promoters.So far, many native, artificial, and engineered promoters have successfully been used for the expression of recombinant proteins in B. subtilis, they are listed as follows.

Given that there is no need for us to express the protein at a particular time, constitutive promoters may be a decent option in consideration of time and cost. Thus, we chose the P43 promoters at first because it’s easiest to obtain and can meet our fundamental requirements.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]