Part:BBa_K4227026:Design

P_nlmC-xip-P34-sigma28

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

After an engineering cycle, we failed in verification the muB. And the modeling shows that the express level of the muB is much lower than other proteins.

In order to have higher level of the expression, we learned the literature and choose the xip-P34,a kind of short peptide which only contains 9 aa.

Source

Synthesized by the Tsingke Biotechnology Co., Ltd.

References

[1] Tianlei Liu, Shoubin Xue, Wenbo Cai, Xiaojing Liu, Xiaojuan Liu, Rongrong Zheng, Hongyan Luo, Wenhui Qi. ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Annals of Microbiology.2013;1 (64) : 31-38.

[2] R Lutz. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research.1997;6 (25) : 1203-1210.

[3] Chowdhury Raihan Bikash, Yftah Tal-Gan. Structure Activity Relationship Study of the XIP Quorum Sensing Pheromone in Streptococcus mutans Reveal Inhibitors of the Competence Regulon. ACS Chemical Biology.2020;10 (15) : 2833-2841.

Sequence and Features