Coding

Part:BBa_K4227001:Design

Designed by: Yang Chen   Group: iGEM22_ZJUT-China   (2022-09-29)


MuB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 493
    Illegal EcoRI site found at 1462
    Illegal EcoRI site found at 2997
    Illegal PstI site found at 1411
    Illegal PstI site found at 1712
    Illegal PstI site found at 2024
    Illegal PstI site found at 2587
    Illegal PstI site found at 3311
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 493
    Illegal EcoRI site found at 1462
    Illegal EcoRI site found at 2997
    Illegal NheI site found at 937
    Illegal PstI site found at 1411
    Illegal PstI site found at 1712
    Illegal PstI site found at 2024
    Illegal PstI site found at 2587
    Illegal PstI site found at 3311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 493
    Illegal EcoRI site found at 1462
    Illegal EcoRI site found at 2997
    Illegal BglII site found at 237
    Illegal BglII site found at 500
    Illegal BglII site found at 2798
    Illegal BglII site found at 3570
    Illegal BamHI site found at 3179
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 493
    Illegal EcoRI site found at 1462
    Illegal EcoRI site found at 2997
    Illegal PstI site found at 1411
    Illegal PstI site found at 1712
    Illegal PstI site found at 2024
    Illegal PstI site found at 2587
    Illegal PstI site found at 3311
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 493
    Illegal EcoRI site found at 1462
    Illegal EcoRI site found at 2997
    Illegal PstI site found at 1411
    Illegal PstI site found at 1712
    Illegal PstI site found at 2024
    Illegal PstI site found at 2587
    Illegal PstI site found at 3311
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 1527
    Illegal NgoMIV site found at 2526
    Illegal NgoMIV site found at 2815
    Illegal NgoMIV site found at 2893
    Illegal NgoMIV site found at 3367
    Illegal NgoMIV site found at 3712
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

Synthesized by the Tsingke Biotechnology Co., Ltd.

References

Nobuyuki Sumitomo, Katsuhisa Saeki, Katsuya Ozaki, Susumu Ito, Tohru Kobayashi. Mutanase from a Paenibacillus isolate: Nucleotide sequence of the gene and properties of recombinant enzymes. Elsevier BV.2007;4 (1770) : 716-724.