Plasmid

Part:BBa_K4227000:Design

Designed by: Yang Chen   Group: iGEM22_ZJUT-China   (2022-09-18)


p7z6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2232
    Illegal XbaI site found at 2259
    Illegal PstI site found at 2778
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2232
    Illegal PstI site found at 2778
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2232
    Illegal BamHI site found at 2253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2232
    Illegal XbaI site found at 2259
    Illegal PstI site found at 2778
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2232
    Illegal XbaI site found at 2259
    Illegal PstI site found at 2778
    Illegal NgoMIV site found at 2630
    Illegal NgoMIV site found at 2691
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

From our PI Junping zhou

References

Xian Zhang, Ruiqi Lu, Qiang Wang, Mengkai Hu, Zhiyue Li, Meijuan Xu, Taowei Yang, Rongzhen Zhang, Zhiming Rao. Production of d-Tagatose by Whole-Cell Conversion of Recombinant Bacillus subtilis in the Absence of Antibiotics. MDPI AG.2021;12 (10) : 1343.