Plasmid

Part:BBa_K4227000

Designed by: Yang Chen   Group: iGEM22_ZJUT-China   (2022-09-18)


p7z6

A plasmid with the lox71-zeocinR-lox66 cassette.

Be used to provide the lox71 and lox66 site in the cre/loxp sysytem in the Bacillus Subtilis

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2232
    Illegal XbaI site found at 2259
    Illegal PstI site found at 2778
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2232
    Illegal PstI site found at 2778
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2232
    Illegal BamHI site found at 2253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2232
    Illegal XbaI site found at 2259
    Illegal PstI site found at 2778
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2232
    Illegal XbaI site found at 2259
    Illegal PstI site found at 2778
    Illegal NgoMIV site found at 2630
    Illegal NgoMIV site found at 2691
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None