Composite
katGp-RFP

Part:BBa_K4225005:Design

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-07)


RFP that is induced by katGp, a H2O2 inducible promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 160
    Illegal BglII site found at 231
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 160
    Illegal AgeI site found at 949
    Illegal AgeI site found at 1061
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Prefix and Suffix are added when we are designing our genes. However, they aren't submitted into the sequence above. However, spacers are added between the parts and is in the sequence above.

Note that, for teams that are planning to digest this composite with RFC[10] enzymes, please use XbaI, SpeI and PstI enzymes as there is an EcoRI cutsite in katGp.