Composite
Pc-OxyR

Part:BBa_K4225000:Design

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-07)


oxyR with constitutive promoter, RBS and 6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Prefix-Suffix are added to our sequence and spacers are also added between the parts. This composite part is also codon optimized for our chassis, DH5α.

Source

References