Composite

Part:BBa_K4223020:Design

Designed by: RongKai Tang   Group: iGEM22_HainanU_China   (2022-09-30)


A protein expression system of Cas13a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1597
    Illegal PstI site found at 2452
    Illegal PstI site found at 3391
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4019
    Illegal PstI site found at 1597
    Illegal PstI site found at 2452
    Illegal PstI site found at 3391
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 338
    Illegal BglII site found at 878
    Illegal BglII site found at 1814
    Illegal BglII site found at 2024
    Illegal BglII site found at 2078
    Illegal BglII site found at 2309
    Illegal BglII site found at 2582
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1597
    Illegal PstI site found at 2452
    Illegal PstI site found at 3391
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1597
    Illegal PstI site found at 2452
    Illegal PstI site found at 3391
    Illegal NgoMIV site found at 2530
    Illegal NgoMIV site found at 3166
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

1

References