Coding
Part:BBa_K4220011:Design
Designed by: Xiaoya Liu Group: iGEM22_NEU_CHINA (2022-09-25)
alpha factor sp-V5-RTAC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 471
Illegal XbaI site found at 1404
Illegal PstI site found at 321
Illegal PstI site found at 760
Illegal PstI site found at 2132 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 321
Illegal PstI site found at 760
Illegal PstI site found at 2132 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1407
Illegal BamHI site found at 2288
Illegal XhoI site found at 2279 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 471
Illegal XbaI site found at 1404
Illegal PstI site found at 321
Illegal PstI site found at 760
Illegal PstI site found at 2132 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 471
Illegal XbaI site found at 1404
Illegal PstI site found at 321
Illegal PstI site found at 760
Illegal PstI site found at 2132
Illegal AgeI site found at 2391 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 283
Illegal BsaI site found at 1604
Illegal BsaI.rc site found at 1292
Design Notes
To express RTAC in the yeast, signal peptide will be replaced with yeast alpha factor signaling peptide.
Source
alpha factor sp: yeast RTAC: mammalian