DNA
Part:BBa_K4218015:Design
Designed by: Wuhui Jiang Group: iGEM22_YiYe-China (2022-10-03)
ecessive exon of MAP3K7-mCherry
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 464
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 464
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 464
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 464
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
During normal splicing of the cell, the internally shifted cryptic exon will be removed, mCherry will be expressed. Because the stop codon was located in front of the GFP gene, GFP could not be translatable and red fluorescent could be detected in cells. In the case where splicing is inhibited, the internally shifted recessive exon will not be removed, the mCherry gene is shifted.
Source
Ecessive exon is form MAP3K7 gene.MCherry is a red fluorescent dye that is widely used in biotechnology as a tracer, ,which isolated from the Discosoma species.We added a A shifted in-frame termination codon (TGATG) at the end of the mcherry sequence.