DNA

Part:BBa_K4218015

Designed by: Wuhui Jiang   Group: iGEM22_YiYe-China   (2022-10-03)


ecessive exon of MAP3K7-mCherry

According to previous studies, we found that exons of mitogen-activated protein kinase kinase kinase 7 (MAP3K7) was skipped in MDS patients. This mechanism gives rise to our idea of constructing the sensor based on the exon skipping of MAP3K7 to the plasmid containing reporter genes, which monitor the alteration of RNA splicing in cells. Our project will help us to evaluate the effectiveness of these sensors in diagnosing MDS patients in the future.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 464
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 464
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 464
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 464
  • 1000
    COMPATIBLE WITH RFC[1000]


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