Composite

Part:BBa_K4218006:Design

Designed by: Wuhui Jiang   Group: iGEM22_YiYe-China   (2022-10-08)


The GFP-mCherry reporter system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1126
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

According to previous studies, we found that exons of mitogen-activated protein kinase kinase kinase 7 (MAP3K7) was skipped in MDS patients. This mechanism gives rise to our idea of constructing the sensor based on the exon skipping of MAP3K7 to the plasmid containing reporter genes, which monitor the alteration of RNA splicing in cells. Our project will help us to evaluate the effectiveness of these sensors in diagnosing MDS patients in the future.


Source

Uses a positively regulated promoter（BBa_I712004），our basic part BBa_K4218015, a strong reporter EGFP(BBa_I714891).

References