Plasmid_Backbone

Part:BBa_K4216045:Design

Designed by: Yves Loiseau--Marchand   Group: iGEM22_Paris_Bettencourt   (2022-10-11)


pCOLADuet_Bettencourt


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1888
    Illegal SpeI site found at 2120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1238
    Illegal NheI site found at 2145
    Illegal NheI site found at 2168
    Illegal SpeI site found at 2120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1888
    Illegal SpeI site found at 2120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1888
    Illegal SpeI site found at 2120
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 223
    Illegal BsaI site found at 3136
    Illegal BsaI.rc site found at 2101


Design Notes

N/A


Source

synthetic DNA and pDUET vectors

References