Coding
Part:BBa_K4214002:Design
Designed by: Jol Tekoniemi Group: iGEM22_Stockholm (2022-10-08)
hGIF with 6xHis-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 257
Illegal BsaI.rc site found at 937
Illegal SapI site found at 927
Design Notes
This part has the signal peptide from BBa_K4214001 removed, as it is not required for bacterial expression. This removal was performed by using primers that excluded the signal peptide.
Source
Human genome, chromosome 11q12.1