Coding

Part:BBa_K4214002:Design

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-08)


hGIF with 6xHis-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 257
    Illegal BsaI.rc site found at 937
    Illegal SapI site found at 927


Design Notes

This part has the signal peptide from BBa_K4214001 removed, as it is not required for bacterial expression. This removal was performed by using primers that excluded the signal peptide.


Source

Human genome, chromosome 11q12.1

References