![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4214001:Design
Designed by: Jol Tekoniemi Group: iGEM22_Stockholm (2022-10-07)
Gastric Intrinsic Factor with signal peptide and polyhistidine tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 21
Illegal SpeI site found at 94
Illegal PstI site found at 1316 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 94
Illegal PstI site found at 1316 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 21
Illegal SpeI site found at 94
Illegal PstI site found at 1316 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 21
Illegal SpeI site found at 94
Illegal PstI site found at 1316
Illegal NgoMIV site found at 14 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 353
Illegal BsaI.rc site found at 1033
Illegal SapI site found at 1023
Design Notes
We incorporated a His-tag and cloning sites
Source
Human genome