Plasmid

Part:BBa_K4213058:Design

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-28)


pDGB3alpha1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 365
    Illegal EcoRI site found at 625
    Illegal XbaI site found at 392
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 365
    Illegal EcoRI site found at 625
    Illegal NheI site found at 3744
    Illegal PstI site found at 404
    Illegal NotI site found at 1619
    Illegal NotI site found at 3151
    Illegal NotI site found at 4441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 365
    Illegal EcoRI site found at 625
    Illegal BamHI site found at 386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 365
    Illegal EcoRI site found at 625
    Illegal XbaI site found at 392
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 365
    Illegal EcoRI site found at 625
    Illegal XbaI site found at 392
    Illegal PstI site found at 404
    Illegal NgoMIV site found at 1064
    Illegal NgoMIV site found at 1238
    Illegal NgoMIV site found at 1288
    Illegal NgoMIV site found at 1292
    Illegal NgoMIV site found at 1319
    Illegal NgoMIV site found at 1818
    Illegal NgoMIV site found at 2176
    Illegal AgeI site found at 4238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 604
    Illegal BsaI.rc site found at 22
    Illegal SapI site found at 4832
    Illegal SapI.rc site found at 4369


Design Notes

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Source

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References