Composite

Part:BBa_K4213030:Design

Designed by: Ioannis Retalis   Group: iGEM22_Thessaly   (2022-08-28)


pNOS:Venus:TetR:NLS:tNOS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 211
    Illegal NheI site found at 365
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1803
    Illegal BsaI.rc site found at 2311


Design Notes

Designed for the observation of the expression and intracellular localization of the TetR protein.

Source

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References