Coding
OsPHT1;6

Part:BBa_K4213021:Design

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-08-27)


PHT1;6 from O. sativa (optimized & domesticated) - linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 512
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 577
    Illegal PstI site found at 512
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 986
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 512
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 512
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-Subpart of Kozak frame added -Codon optimization for N.benthamiana


Source

Oryza sativa

References