Plasmid_Backbone

Part:BBa_K4212064:Design

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-10-03)


STK-0-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 450
    Illegal XbaI site found at 465
    Illegal PstI site found at 1507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 450
    Illegal PstI site found at 1507
    Illegal NotI site found at 456
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 450
    Illegal XhoI site found at 326
    Illegal XhoI site found at 2524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 450
    Plasmid lacks a suffix.
    Illegal PstI site found at 1507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 450
    Plasmid lacks a suffix.
    Illegal XbaI site found at 465
    Illegal PstI site found at 1507
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 661


Design Notes

sfGFP dropout cassette inserted within the MCS of backbone, flanking with BsaI and BsmBI sites. Dropout cassette is replaced by target sequences, which enables to verify the insertion of target sequences if transformants do not express sfGFP.


Source

Synthetic construct.

References