Plasmid_Backbone
Part:BBa_K4212064:Design
Designed by: Youzi Bi Group: iGEM22_Imperial_College_London (2022-10-03)
STK-0-sfGFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 450
Illegal XbaI site found at 465
Illegal PstI site found at 1507 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 450
Illegal PstI site found at 1507
Illegal NotI site found at 456 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 450
Illegal XhoI site found at 326
Illegal XhoI site found at 2524 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 450
Plasmid lacks a suffix.
Illegal PstI site found at 1507 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 450
Plasmid lacks a suffix.
Illegal XbaI site found at 465
Illegal PstI site found at 1507 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 661
Design Notes
sfGFP dropout cassette inserted within the MCS of backbone, flanking with BsaI and BsmBI sites. Dropout cassette is replaced by target sequences, which enables to verify the insertion of target sequences if transformants do not express sfGFP.
Source
Synthetic construct.