Plasmid

Part:BBa_K4212046:Design

Designed by: Yuancheng Ding   Group: iGEM22_Imperial_College_London   (2022-09-30)


SDP3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3856
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3856
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3856
    Illegal BglII site found at 1221
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3856
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3856
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

According to the website Doulix, the double terminator B0015 was created by iGEM registry. It is one of the most widely used terminators. The Cas9 protein is one of the most crucial part in CRISPR technology, which can cut foreign DNA efficiently (Jinek et al., 2012). We included it in our self-digesting plasmid construct. Spacers were created to maximize the ligation fidelity in the final assembly.


Source

Synthetic construct.

References

[1] https://parts.igem.org/Part:BBa_B0015:Design [2] https://doulix.com/biomodules/62PCZ1Z/ [3] Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A. & Charpentier, E. (2012) A programmable dual RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (New York, N.Y.). 337 (6096), 816–821. doi:10.1126/science.1225829.